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mouse anti myo7a monoclonal antibody (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank mouse anti myo7a monoclonal antibody
Mouse Anti Myo7a Monoclonal Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 48 article reviews

mouse anti myo7a monoclonal antibody - by Bioz Stars, 2026-03

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Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery

Journal: Journal of Biomedical Science

Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

doi: 10.1186/s12929-025-01180-4

Figure Lengend Snippet: Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery

Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

Techniques: RNA Sequencing

Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )

Journal: Journal of Biomedical Science

Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

doi: 10.1186/s12929-025-01180-4

Figure Lengend Snippet: Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )

Techniques: Expressing, Confocal Microscopy, Control, Fluorescence

Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test

Journal: Journal of Biomedical Science

Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

doi: 10.1186/s12929-025-01180-4

Figure Lengend Snippet: Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test

Techniques: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence

Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test

Journal: Journal of Biomedical Science

Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

doi: 10.1186/s12929-025-01180-4

Figure Lengend Snippet: Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test

Techniques: Expressing, In Vitro, Control, RNAscope, Immunohistochemistry, Fluorescence

Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test

Journal: Journal of Biomedical Science

Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

doi: 10.1186/s12929-025-01180-4

Figure Lengend Snippet: Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test

Techniques: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence

KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. Myosin7a antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.

Journal: iScience

Article Title: mTORC2 regulates auditory hair cell structure and function

doi: 10.1016/j.isci.2023.107687

Figure Lengend Snippet: KO confirmation of HC-RicKO mice (A) Hemizygous Myo15 - Cre +/− mice were crossed with homozygous Rictor fl/fl mice harboring LoxP sites upstream and downstream of exon 4 and 5, respectively. Forward (F) and reverse primers (R) were used to detect Cre-mediated recombination, resulting in a 280 bp PCR product after Cre-mediated recombination of the Rictor allele. (B) PCR using genomic DNA from dissected organ of Corti (OC), spiral ganglion (SG) or vestibular organ (vest; macular and cristae ampullaris organs pooled) tissue, separate for left (L) and right (R) inner ears. Genomic DNA from outer ear skin of both genotypes was used as negative control of Cre-mediated recombination. Genomic DNA from heart samples of tamoxifen-induced cardiomyocyte-specific Rictor KO mice was used as positive control (pos ctrl). No template control (NTC) contains all PCR reaction components including primers but no DNA. Successful Cre-mediated recombination of the Rictor allele was only found in OCs and vestibular organs of HC-RicKO mice. (C) Representative images (maximum intensity projections) of the medial cochlear turn from 4-week-old mice stained with an antibody against Akt-pSer473. Myosin7a antibody, phalloidin and nuclear DAPI staining visualize the hair cells. Scale bar for all figures = 20 μm. (D) Quantification of mean fluorescence intensity (MFI) in arbitrary units (a.u.) of the Akt-pSer473 signal intensity in the medial cochlear turn of 4-week-old mice. n = 3 mice per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05.

Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

Techniques: Negative Control, Positive Control, Staining, Fluorescence

Hearing loss precedes hair cell loss in HC-RicKO mice (A) Cochleograms showing inner hair cell (left graphs) and outer hair cell (right graphs) loss in 5 percent (%) distances from the Apex at indicated timepoints. Place-frequency map calculated with formula d = (LOG10((f+6.664)/9.8)/LOG10(10))/0.0092 (where d is the distance from the Apex in % and f the frequency in kHz). , , n = 3–4 mice (2 weeks), 4 mice (4 weeks), 3 mice (8 weeks), and 3–4 mice (12 weeks) per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (B) Representative images (maximum intensity projections) of the medial cochlear turn from 8-week-old mice of both genotypes. Hair cells are visualized with phalloidin, a Myosin7a antibody and nuclear DAPI staining. Scale bar for all figures = 20 μm.

Journal: iScience

Article Title: mTORC2 regulates auditory hair cell structure and function

doi: 10.1016/j.isci.2023.107687

Figure Lengend Snippet: Hearing loss precedes hair cell loss in HC-RicKO mice (A) Cochleograms showing inner hair cell (left graphs) and outer hair cell (right graphs) loss in 5 percent (%) distances from the Apex at indicated timepoints. Place-frequency map calculated with formula d = (LOG10((f+6.664)/9.8)/LOG10(10))/0.0092 (where d is the distance from the Apex in % and f the frequency in kHz). , , n = 3–4 mice (2 weeks), 4 mice (4 weeks), 3 mice (8 weeks), and 3–4 mice (12 weeks) per genotype. Results are presented as means ± SDs. Student’s t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (B) Representative images (maximum intensity projections) of the medial cochlear turn from 8-week-old mice of both genotypes. Hair cells are visualized with phalloidin, a Myosin7a antibody and nuclear DAPI staining. Scale bar for all figures = 20 μm.

Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

Techniques: Staining

Journal: iScience

Article Title: mTORC2 regulates auditory hair cell structure and function

doi: 10.1016/j.isci.2023.107687

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-myosin7a IgG1 , Developmental Studies Hybridoma Bank , Cat#138-1s; RRID: AB_2282417.

Techniques: Recombinant, Saline, Electron Microscopy, Software, Imaging

(A) Overview of inner ear organoid differentiation. (B) Bright-field images of cell aggregates at different time points for two hiPSC lines showing morphological differences. Representative images from ≥8 aggregates (≥3 experiments). Scale bars, 500 μm. (C) Representative H&E and IHC images of D21 aggregates containing otic vesicles (asterisk) expressing TFAP2A, CDH1, SOX10, SIX1, and SOX2. Scale bars: H&E, 100 μm; IHC, 50 μm. (D) H&E and IHC images of D67 aggregates showing an IEO with epithelial vesicles (asterisks, CDH1 + , SOX10 + ), containing hair cells (MYO7A + ) and neurons (TUBB3 + ). Scale bars: H&E, 100 μm; IHC, 50 μm. (E) Epithelial thickness measurements of D3 aggregates treated with different BMP-4 concentrations at D0 for two cell lines. Efficient IEO induction achieved at 1.25 ng/mL BMP-4 (“Optimal”). Statistical significance analyzed using 2-way ANOVA with Šidák correction. Data considered significant if p < 0.05. n = 10 per datapoint shown in a min-max boxplot. Representative graph from two experiments. CNCCs, cranial neural crest cells; D, differentiation day; IEO, inner ear organoid; OEPD, otic-epibranchial placode domain. ****p ≤ 0.0001. H&E and IHC images (C and D) are representative of ≥6 aggregates (≥2 experiments). See also .

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet: (A) Overview of inner ear organoid differentiation. (B) Bright-field images of cell aggregates at different time points for two hiPSC lines showing morphological differences. Representative images from ≥8 aggregates (≥3 experiments). Scale bars, 500 μm. (C) Representative H&E and IHC images of D21 aggregates containing otic vesicles (asterisk) expressing TFAP2A, CDH1, SOX10, SIX1, and SOX2. Scale bars: H&E, 100 μm; IHC, 50 μm. (D) H&E and IHC images of D67 aggregates showing an IEO with epithelial vesicles (asterisks, CDH1 + , SOX10 + ), containing hair cells (MYO7A + ) and neurons (TUBB3 + ). Scale bars: H&E, 100 μm; IHC, 50 μm. (E) Epithelial thickness measurements of D3 aggregates treated with different BMP-4 concentrations at D0 for two cell lines. Efficient IEO induction achieved at 1.25 ng/mL BMP-4 (“Optimal”). Statistical significance analyzed using 2-way ANOVA with Šidák correction. Data considered significant if p < 0.05. n = 10 per datapoint shown in a min-max boxplot. Representative graph from two experiments. CNCCs, cranial neural crest cells; D, differentiation day; IEO, inner ear organoid; OEPD, otic-epibranchial placode domain. ****p ≤ 0.0001. H&E and IHC images (C and D) are representative of ≥6 aggregates (≥2 experiments). See also .

Article Snippet: Mouse monoclonal anti-MYO7A (1:30) , DSHB , Cat#: 138-1s; RRID: AB_2282417.

Techniques: Expressing, IF-P

(A) UMAP plot of annotated human inner ear atlas highlighting mesenchymal cell types. (B) UMAP plot comparing reference POM (left) and mapped IEO-derived POM population (right) using Symphony. (C) Density plots showing expression of epithelium-associated markers in the IEO-derived POM population. (D) OTOR + POU3F4 + mesenchymal cells surrounding IEO-vesicles containing MYO7A + hair cells at D75. Scale bar, 50 μm. (E) OTOR + mesenchymal cells beneath fetal utricle sensory epithelium (FW10.6). Scale bar, 20 μm. POM, periotic mesenchyme. IHC images are representative of n ≥ 6 aggregates (≥2 experiments) (D) or n ≥ 2 human inner ear tissues of matching fetal age (E).

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet: (A) UMAP plot of annotated human inner ear atlas highlighting mesenchymal cell types. (B) UMAP plot comparing reference POM (left) and mapped IEO-derived POM population (right) using Symphony. (C) Density plots showing expression of epithelium-associated markers in the IEO-derived POM population. (D) OTOR + POU3F4 + mesenchymal cells surrounding IEO-vesicles containing MYO7A + hair cells at D75. Scale bar, 50 μm. (E) OTOR + mesenchymal cells beneath fetal utricle sensory epithelium (FW10.6). Scale bar, 20 μm. POM, periotic mesenchyme. IHC images are representative of n ≥ 6 aggregates (≥2 experiments) (D) or n ≥ 2 human inner ear tissues of matching fetal age (E).

Article Snippet: Mouse monoclonal anti-MYO7A (1:30) , DSHB , Cat#: 138-1s; RRID: AB_2282417.

Techniques: Derivative Assay, Expressing

(A) UMAP plot of annotated human inner ear atlas highlighting hair cells (HCs). (B) UMAP plot comparing reference hair cell population (left) and mapped IEO-derived hair cell population (right) using Symphony. (C) Density plots of reference hair cell population (left) for immature, type I, and type II vestibular markers, and density plots of mapped IEO-derived hair cells showing expression of immature markers. (D) Schematic depicting morphological differences between type I and type II vestibular hair cells. (E) Calyx-type synapse (TUBB3 + ) surrounding a hair cell (MYO7A + ). Scale bar, 10 μm. (F) Expression of ANXA4 and OCM in hair cells of a D75 IEO, showing subsets with none, either, or both. Scale bar, 10 μm. (G) OCM expression in hair cells of human fetal utricle (FW10.6). Scale bar, 10 μm. (H) ANXA4 expression in human fetal utricle (FW10.6). Scale bar, 10 μm. (I) Methylene blue-azure II staining of a D74 IEO-vesicle containing hair cells (arrow and “HC”). Scale bar, 20 μm. (J) Hair bundle with stereocilia and kinocilium cross-section (arrow). Scale bar, 1 μm. (K) Apical region of a hair cell with cuticular plate (yellow) and stereociliary rootlets. Scale bar, 1 μm. (L) Basal region of a hair cell showing synaptic ribbons (arrow) and bouton-like synapses (“N”). Scale bar, 500 nm. (M) Transection of type I vestibular hair cell almost entirely surrounded by calyx-type synapse (in red). Scale bar, 500 nm. (N) OTOF expression in MYO7A + hair cells. Scale bar, 10 μm. (O) H&E overview with CDH23 in stereocilia by IHC. Scale bars: H&E, 100 μm; IHC, 20 μm. (P) H&E overview with WHRN in stereocilia by IHC. Scale bars: H&E, 100 μm; IHC, 20 μm. H&E and IHC images are representative of n ≥ 6 aggregates (≥2 experiments) (E, F, and N–P) or n ≥ 2 human inner ear tissues of matching fetal age (G and H). TEM images are representative of n R 3.

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet: (A) UMAP plot of annotated human inner ear atlas highlighting hair cells (HCs). (B) UMAP plot comparing reference hair cell population (left) and mapped IEO-derived hair cell population (right) using Symphony. (C) Density plots of reference hair cell population (left) for immature, type I, and type II vestibular markers, and density plots of mapped IEO-derived hair cells showing expression of immature markers. (D) Schematic depicting morphological differences between type I and type II vestibular hair cells. (E) Calyx-type synapse (TUBB3 + ) surrounding a hair cell (MYO7A + ). Scale bar, 10 μm. (F) Expression of ANXA4 and OCM in hair cells of a D75 IEO, showing subsets with none, either, or both. Scale bar, 10 μm. (G) OCM expression in hair cells of human fetal utricle (FW10.6). Scale bar, 10 μm. (H) ANXA4 expression in human fetal utricle (FW10.6). Scale bar, 10 μm. (I) Methylene blue-azure II staining of a D74 IEO-vesicle containing hair cells (arrow and “HC”). Scale bar, 20 μm. (J) Hair bundle with stereocilia and kinocilium cross-section (arrow). Scale bar, 1 μm. (K) Apical region of a hair cell with cuticular plate (yellow) and stereociliary rootlets. Scale bar, 1 μm. (L) Basal region of a hair cell showing synaptic ribbons (arrow) and bouton-like synapses (“N”). Scale bar, 500 nm. (M) Transection of type I vestibular hair cell almost entirely surrounded by calyx-type synapse (in red). Scale bar, 500 nm. (N) OTOF expression in MYO7A + hair cells. Scale bar, 10 μm. (O) H&E overview with CDH23 in stereocilia by IHC. Scale bars: H&E, 100 μm; IHC, 20 μm. (P) H&E overview with WHRN in stereocilia by IHC. Scale bars: H&E, 100 μm; IHC, 20 μm. H&E and IHC images are representative of n ≥ 6 aggregates (≥2 experiments) (E, F, and N–P) or n ≥ 2 human inner ear tissues of matching fetal age (G and H). TEM images are representative of n R 3.

Article Snippet: Mouse monoclonal anti-MYO7A (1:30) , DSHB , Cat#: 138-1s; RRID: AB_2282417.

Techniques: Derivative Assay, Expressing, Staining

(A) Ligand-receptor interactions in NECTIN, NOTCH, and BMP signaling pathways. Validated in human inner ear atlas (FW7.5 and/or FW9.2). (B) Heatmap showing total interactions between cell types. x axis, receivers (with receptors); y axis, senders (with ligands). (C) PECAM1 + endothelial cells underlying sensory epithelium with MYO7A + hair cells in D75 IEO (upper) and FW9.2 fetal utricle (lower). Images are representative of n ≥ 6 aggregates (≥2 experiments) or n ≥ 2 human inner ear tissue with matching fetal age. Scale bars, 50 μm. (D) Chord diagrams displaying interactions between cell types in IEOs. Arrows indicate sender to receiver cell types. POM, periotic mesenchyme. See also and .

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet: (A) Ligand-receptor interactions in NECTIN, NOTCH, and BMP signaling pathways. Validated in human inner ear atlas (FW7.5 and/or FW9.2). (B) Heatmap showing total interactions between cell types. x axis, receivers (with receptors); y axis, senders (with ligands). (C) PECAM1 + endothelial cells underlying sensory epithelium with MYO7A + hair cells in D75 IEO (upper) and FW9.2 fetal utricle (lower). Images are representative of n ≥ 6 aggregates (≥2 experiments) or n ≥ 2 human inner ear tissue with matching fetal age. Scale bars, 50 μm. (D) Chord diagrams displaying interactions between cell types in IEOs. Arrows indicate sender to receiver cell types. POM, periotic mesenchyme. See also and .

Article Snippet: Mouse monoclonal anti-MYO7A (1:30) , DSHB , Cat#: 138-1s; RRID: AB_2282417.

Techniques:

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-MYO7A (1:30) , DSHB , Cat#: 138-1s; RRID: AB_2282417.

Techniques: Transduction, Recombinant, RNA Sequencing Assay, Software, Cell Attachment Assay

Journal: eLife

Article Title: A gradient of Wnt activity positions the neurosensory domains of the inner ear

doi: 10.7554/eLife.59540

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal IgG1 anti-Myo7a (RRID: AB_2282417 ) , Developmental Studies Hybridoma Bank , Clone 138–1 , IF (1:500).

Techniques: Software, Recombinant, Plasmid Preparation, Clone Assay, Control, Cloning, Isolation, Concentration Assay, Sequencing

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